Involvement of stromal cell-derived factor-1α(SDF-1α),stem cell factor(SCF) and fractalkine(FKN) in Ginsenoside Rg1 protection against intimal hyperplasia in rat balloon injury
作者单位：Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnocentric of Ministry of EducationZunyi Medical University
会议名称：《首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会》
会议日期：2018年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Balloon injury TSG SCF/c-kit SDF-1α/CXCR4 FKN/CX3CR1 Intimal hyperplasia
摘 要：Background:Intimal hyperplasia is the major therapeutic concern after percutaneous coronary *** aim of this study is to investigate effects of Ginsenoside Rgl on intimal hyperplasia and the underling mechanisms through attenuating the expressions of stromal cell-derived factor-1α(SDF-1α)/CXCR4,stem cell factor(SCF)/c-kit and fractalkine(FKN)/CX3 ***:Rats were operated with carotid artery balloon *** treatment groups were gavaged with 8 and 16 mg/kg/d of Ginsenoside *** 14 days of treatment,carotid artery pathological changes were evaluated by *** expression ofα-SMA and PCNA were detected by double-label *** levels of SDF-1α,SCF and FKN were detected by enzyme linked immunosorbent *** protein expressions of SCF,SDF-1α and FKN,as well as the receptors c-kit,CXCR4 and CX3 CR1 were detected by ***:Ginsenoside Rg1 dose-dependently inhibited balloon injury-induced intimal hyperplasia,as evidenced by reducing neointima area(NIA),neointima area/media area(NIA/MA),neointima area/internal elastic area(NIA/IELA),and decreasing the protein expression of α-SMA and *** Rg1 reduced serum levels of SDF-1α,SCF and FKN, and it also decreased the protein expressions of SCF, SDF-1αand FKN,as well as the receptors c-kit,CXCR4 and CX3 ***:This study clearly demonstrated that Ginsenoside Rg1 was effective in inhibiting intimal hyperplasia,and this effect was mediated,at least in part,through the SCF/c-kit,SDF-1α/CXCR4 and FKN/CX3 CR1 axes.