Effects on drought stress of relative species of sweetpotato physiological and biochemical characteristics and gene expression profile
作者单位：The College of life Science Jiangsu Normal University Jiangsu Xuzhou Sweetpotato Research Center/Sweetpotato Research Institute CAAS/Key Laboratory for Biology and Genetic Breeding of Sweetpotato Ministry of Agriculture
会议名称：《江苏省遗传学会第九次会员代表大会暨学术研讨会》
会议日期：2015年
关 键 词：sweetpotao(Ipomoea trifida) PEG-6000 drought stress Physiological and biochemical characteristics gene expression RNA-seq
摘 要：Drought stress is in agricultural production in china even all over the world are often facing the worldwide problem,make the output of crop production and quality are limited,so far this problem,water stress has become the current must solve the problem. Ipomoea trifida（Kunth） G. Don（2 n=2 x=30） is one of the ancestors of a sweetpotato, high-drought resistance. However, the drought-resistant physiological mechanism is still not reported. In this study, by the method of the simulated-drought water culture, with 30% concentrations of PEG-6000, effects of drought stress on wild relatives of sweetpotato seedling leaf physiological and biochemical indexes. The results showed that the malonaldehyde（MDA） and free proline（Pro） content increased with the prolongation of PEG-6000 treatment time. Peroxidase（POD）, catalase（CAT） and glutathione reductase（GR） activity were enhanced after PEG treatment, The content of chlorophyll was decreased and the content of soluble sugar increased, and the relative conductivity increased slowly compared with the control,which reveals different changing law case in the wild relatives of sweetpotato MDA and Pro content and enzyme activity in a short period of acute stress, which can provide some references for screening drought resistant varieties and tapping drought-resistant related *** is one of the most important food crops in the world, it is also the new energy crops, but drought problems limit the yield and the geographical distribution of crops, molecular mechanism for drought stress response of the sweet potato, we use illumina hiseq2000 for transcriptome sequencing to suffer under drought stress large sweet potato wild species, functional genomic origin to get it the molecular mechanism for drought tolerance and better. Using illumina hiseq2000 sequencing technology on drought stress treatment combination control samples of library construction and sequencing, in this experiment, we usethe illumina hiseq2000 sequencing