WRKY transcription factors regulate petal senescence and associated anthocyanin biosynthesis in Jasminum sambac flowers
作者单位：College of Life Sciences Fujian Agricultrue and Forestry University Fujian Provincial Key Laboratory of Plant Functional Biology College of Life Sciences Fujian Agricultrue and Forestry University
会议名称：《第三届全国植物开花·衰老与采后生物学大会》
会议日期：2019年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：WRKYs Anthocyanin Senescence Jasminum sambac
摘 要：Being a valued aromatic and ornamental plant, Jasminum sambac is widely cultivated in the Southeast Asia for utilization of its flowers. Floral aging in J. sambac starts about 24 hours post anthesis（HPA） and ends up in about 36 HPA, with a visual sign of petal-color-turning from white to violet. UPLC-TOF-MS analysis indicated the accumulation of cyanidin 3-O-rutinoside associated with the aging progression. Floral ethylene and salicylic acid release peaked at c.a. 12 HPA correlating with senescence development, 12 hours ahead of color change. To gain more inside into the gene regulation network in this particular flower aging process, we conduct a transcriptome analysis using total RNA samples from petals at 0, 12, 24 and 36 HPA. We identified several transcription factors which were co-expressed well with anthocyanin biosynthesis and ethylene and salicylic acid biosynthesis/responsive genes. These included one MYC gene, two bHLH genes and three WRKY genes among others. We isolated the full-length cDNA sequences of these genes and further characterized their functions in transgenic J. sambac, petunia and Arabidopsis that together provided primary evidences of a master effect on floral aging by the WRKY transcription factors. Interactions of the three WRKY proteins indicated that they co-operated in the regulations of the senescence processes.