Preliminary study of microRNAs related to early mycosis fungoides
作者单位：Department of DermatologyChinese Academy of Medical Sciences and Peking Union Medical College Institute of DermatologyChinese Academy of Medical Sciences and peking Union Medical College
会议名称：《江苏省遗传学会2016年学术年会》
会议日期：2016年
学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学]
基 金：supported by the innovation fund of Peking Union Medical College(2014-10023-1002-3010) the Natural Science Foundation of the Jiangsu Bureau of Science and Technology(BK2012505,BK20131063)
关 键 词：Array Early Stage Microrna Mycosis fungoides Myla cell RT-PCR
摘 要：To screen microRNAs（miRNAs） related to early mycosis fungoides（MF）,differentially expressed miRNAs between tissue samples from 6 patients with early MF and 6 control patients with eczema or lichen planus were screened with a high throughout miRNA PCR *** differentially expressed miRNAs were then verified by real-time fluorescentquantitative PCR（RT-qPCR） with tissue samples and Mylacell *** results showed that three differentially expressed miRNAs were showed in the array,which were hsa-miR-378 a-5 p、hsa-miR-302 c-3 p、*** of them were statistically significantly ***-qPCRresults of tissue samples werein accordance with miRNA ***,the RT-qPCR results of Myla cell line showed significantly up-regulated hsa-miR-378 a-5 p and hsa-miR-107,compared with peripheral T lymph cell from a healthy volunteer,hsa-miR-302 c-3 p was not significantly *** are different expression profiles of miRNAsbetween early MF and benign inflammatory dermatoses.