Characterization of the propham biodegradation pathway in Starkeya *** YW6 and cloning of a novel amidase gene mmH
作者单位：Eco-Environmental Protection Research Institute Shanghai Academy of Agricultural Sciences
会议名称：《2019年中国土壤学会土壤环境专业委员会、土壤化学专业委员会联合学术研讨会》
会议日期：2019年
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
关 键 词：MmH Amidase Propham Gene cloning
摘 要：We isolated strain Starkeya ***6,that could degrade *** YW6 metabolizes propham via a pathway in which propham is initially hydrolyzed to aniline and then converted to catechol,which is then oxidized via *** novel amidase gene mmH was cloned from strain YW6 and expressed in *** BL21(DE3).MmH,which exhibits aryl acylamidase activity,was purified for enzymatic *** analysis confirmed that MmH belongs to amidase signature(AS) enzyme family and shares 26-50% identity with several AS family ***(molecular mass of 53 kDa) was most active at 40 oC and pH 8.0 and showed high activity toward *** characteristics make MmH suitable for novel amide biosynthesis and environmental remediation.