Structural mapping on nucleoproteins of nonsegmented negative-sense RNA viruses
作者单位：iHuman Institute ShanghaiTech University
会议名称：《第六届全国冷冻电子显微学与结构生物学专题研讨会》
会议日期：2019年
学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学]
摘 要：The nucleoprotein (N) of non-segmented negative-sense RNA viruses, such as Measles,Ebola and Newcastle disease viruses (NDV), enwrap viral genomic RNAs and assemble them into helical filaments which serve as the template for replication and transcription. Using cryo-Electron Microscopy, we identified and resolved NDV N forming a 4.8?resolution clam-shaped structure with two single-turn spirals packing in a back-to-back mode, which have not been reported before.