A mutated rabbit defensinNP-1 produced by Chlorella ellipsoidea can improve the growth performance of broiler chickens
作者单位：State Key Laboratory of Plant Cell and Chromosome Engineering The Innovative Academy of Seed Design Institute of Genetics and Developmental Biology Chinese Academy of Sciences The 306th Hospital of PLA School of Food andBiological Engineering Jiangsu University Polo Biology Science Park Co. Ltd. College of Life Sciences University of Chinese Academy of Sciences
会议名称：《中国生物工程学会第十三届学术年会暨2019年全国生物技术大会》
会议日期：2019年
学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学]
摘 要：The demand for alternatives to antibiotics to improve the growth performance of food animals is increasing. Defensins constitute the first line of defence against pathogens in the innate immune system of animals and humans. A transgenic Chlorella ellipsoidea strain producing mN P-1(a mutated rabbit defensin NP-1) was previously obtained in our laboratory. In this study, a process for producing the transgenic strain on a large scale was developed, and the C. ellipsoidea strain producing mNP-1 was used as a feed additive to improve the health and growth performance of chickens. The volume of *** producing mNP-1 can be scaled up to 10,000 L with approximately 100 g/L dry biomass, and the mN P-1 content of transgenic microalgal powder(TMP) was 90–105 mg/L. A TMP-to-regular feed ratio of 1‰, as the optimal effective dose, can promote the growth of broiler chickens by increasing weight by 9.27–12.95%. mNP-1 can improve duodenum morphology by promoting long and thin villi and affect the microbial community of the duodenum by increasing the diversity and abundance of beneficial microbes. These results suggested that transgenic Chlorella producing mNP-1 can be industrially produced and used as an effective feed additive and an alternative to antibiotics for improving the health and growth performance of broiler chickens or other types of food animals/poultry.