Detection of trained immunity in rabbits with coccidia infection
作者单位：National Animal Protozoal Laboratory & College of Veterinary MedicineChina Agricultural University
会议名称：《第十一届全国寄生虫学青年工作者学术研讨会》
会议日期：2018年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：China Agriculture Research System-43(CARS-43) and Ministry of Agriculture(948 Program:20015-Z31)
关 键 词：Trained immunity Rabbit Eimeria intestinalis Eimeria magna
摘 要：Recent studies showed that trained immunity,or innate immune memory,demonstrates adaptive characteristics after challenge with pathogens or their products.This new concept leads us to hypothesis that trained immunity plays important role in the protection of rabbits against re-infection with Eimeria,which are the causative agents of rabbit coccidiosis threating the rabbit industry.To test whether Eimeria could stimulate trained immunity in rabbits against homologous and heterologous challenge,two groups of rabbits were individually immunized with 104 E.intestinalis oocysts,then challenged either with 106 E.intestinalis or 106 E.magna oocysts 14 days post immunization.Heparinized blood was sampled each day from day 0 to 9 post immunization(pi) and post challenge(pc).Numbers of white blood cells(WBC),polymorphonuclear neutrophils(PMN) and lymphocytes(LYM) were analyzed.For immunized groups,the numbers of WBC,PMN and LYM increased,and the peak numbers occurred on day 7 to 8 pi with a 2-fold and 1.8-fold higher than those on day 0 pi,respectively.After homologous or heterologous challenge,both WBC and PMN numbers in immunized groups increased with the peak numbers occurred on day 2 and 3 post challenge,respectively;while the LYM numbers increased from day 6 post challenge.Together,the results suggest that immunization with E.intestinalis could induce trained immunity against homologous and heterologous challenge.