Genome-wide association study and transcriptome analysis reveal integrative RNA-and miRNA-profile analysis reveals a likely role of organic nitrogen compound in thickness of pod canopy regulation of ***
作者单位：Academy of Agricultural SciencesSouthwest University
会议名称：《中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会》
会议日期：2018年
关 键 词：transcriptome Brassica napus pod canopy thickness miRNA
摘 要：Oilseed rape(Brassica napus L.) is the second largest oilseed crop worldwide and one of the most important oil crops in *** a component of plant architecture,thickness of pod canopy(TPC) plays an important role in yield performance,especially under high-density planting ***,mechanisms underlying TPC regulation are still largely not *** groups of oilseed rape lines with significantly different TPC were used to conduct RNA-and miRNA-profiling at bolting with tiny flower bud stage,identifying differential expression of a large number of genes involved in nitrogen-and carbon-related *** nitrogen-and carbon-response genes,including ASPS,ASP2,ASN3,ATCYSC1,PAI2,APT2,CRTISO,C0 X15,et *** dramatically enriched in the compact *** expressed miRNAs included those involved in regulating nitrogen-and *** patterns of most target genes were fine-tuned byrelated miRNAs,such as miR159,miR6029,and miR827.12 miRNAs were found to be differentially expressed at both sites,including miR319,miR845,*** results provide insight that nitrogen-and carbon-signaling may play a pivotal role in thickness of pod canopy regulation.