Genome-wide screening and analysis of imprinted genes in rapeseed(Brassica napus L.)endosperm
作者单位：Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture Agriculture and FoodCommonwealth Scientific and Industrial Research Organization
会议名称：《中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会》
会议日期：2018年
关 键 词：rapeseed endosperm RNA sequencing imprinting allele-specific DNA methylation
摘 要：Species-specific genomic imprinting is an epigenetic phenomenon leading to parent-of-origin specific differential expression of maternally and paternally inherited *** date,no studies of imprinting have been reported in rapeseed,a tetraploid ***,we analyzed global patterns of allelic gene expression in developing rapeseed endosperms from reciprocal crosses between inbred lines YN171 and 93275.14 394 genes were found to harbor diagnostic SNPs between the parental lines.A total of 183 imprinted genes,consisting of 167 maternal expressed genes(MEGs) and 16 paternal expressed genes(PEGs) were *** imprinted genes were validated in different endosperm stages and other parental combinations by RT-PCR analysis.A clear clustering of imprinted genes throughout the rapeseed genome was identified,which was different from most other *** analysis to the 104 imprinted genes showed there were 11 genes with differentially methylated regions(DMRs).Unexpectedly,because of lower methylation levels,among the 11 genes,1 gene(BnC04 g0675620) exhibited high CG methylation rate in paternal allele and had big difference between parent *** research on the imprinting mechanism of BnC04 g0675620 needs to be *** results extend our understanding of gene imprinting in plants and provide potential avenues for further research in imprinted genes.