Whole genome re-sequencing of Eimeria tenella reveals positive selection in the precocious lines
作者单位：National Animal Protozoa LaboratoryCollege of Veterinary MedicineChina Agricultural University
会议名称：《第十一届全国寄生虫学青年工作者学术研讨会》
会议日期：2018年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
基 金：The National Natural Science Foundation of China(31330076)
关 键 词：Eimeria tenella re-sequencing precocious positive selection
摘 要：Chicken coccidiosis,caused by Eimeria species,leads to huge economic losses in poultry *** immunization with attenuated live vaccine seems to be the most cost-effective way for controlling this *** produce such attenuated live vaccine,different Eimeria species/strains were selected for precocious lines,which characterized by greatly shorted prepatent time and faster endogenous *** these precocious line-based vaccines are widely used,the genetic mechanisms of the precocity phenomenon remain *** this study,we isolated 19 *** strains from different geographical samples by multiple-oocyst *** the determination of the prepatent time,oocyst output curve,total oocyst output and damage to the chicken intestine(lesion scores) for each strain,5 low-virulent strains(prepatent time 120 h,mean lesion score(1× 104 inoculation) 2) and 14 virulent strains were *** DNA extracted from each strain was used for Illumina sequencing.A total of 112 Gb clean data were produced and the mean coverage was 106×,and 679318 SNPs were detected in *** linkage disequilibrium decay distance of the virulent group was much shorter than the lowvirulent group,which means the low-virulent precocious lines undergo strong positive ***,we found a large select sweep region(260 Kb) in Lachesisroup 9(chromosome in new reference genome) by fixation index(FST) in 10 kb sliding window with a step size of 1 *** protein coding genes under positive selection is under *** results open a window for deciphering the mechanisms of the precocity in Eimeria.