Morphological and mitochondrial genomic characterization of eyeworms(Thelazia callipaeda) from clinical cases in central China
作者单位：Department of ParasitologySchool of Basic Medical SciencesZhengzhou University
会议名称：《第十一届全国寄生虫学青年工作者学术研讨会》
会议日期：2018年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学]
基 金：supported by the Outstanding Young Talent Research Fund of Zhengzhou University(1521328001)
关 键 词：Thelazia callipaeda thelaziasis mitochondrial genome next generation sequencing phylogeny
摘 要：Objective:Thelazia callipaeda,also called the oriental eyeworm,is the major etiological agent of human *** of thelaziasis have increased in recent years in *** this species is of medical importance,the genetics and phylogenetic systematics of *** are poorly ***:In this study,we first reported three cases of thelaziasis in central *** clinical isolates were identified as *** according to morphological characteristics by light microscopy and scanning electron ***,complete mitochondrial(mt) genomes for the three *** isolates from different geographical locations were fully characterized using an Illumina sequencing *** addition,all available mt genomes of spirurid nematodes in Gen Bank were included to reconstruct the phylogeny and to explore the evolutionary histories of the ***:The genome features of the *** isolates contained 12 PCGs,22 transfer RNA genes,two ribosomal RNA genes and a major non-coding *** mt DNA nucleotide sequences of the *** isolates from different hosts and different locations were *** nad6 gene showed high sequence variability among all isolates,which is worth considering for future population genetic studies of *** analyses based on maximum parsimony(MP) and Bayesian inference(BI) methods revealed close relationships among Thelaziidae,Onchocercidae,Setariidae,Gongylonematidae,Physalopteridae,Dracunculidae and *** monophyly of the *** isolates from different hosts and distinct geographical locations was ***:The entire mt genomes of *** presented in this study will serve as a useful dataset for studying the population genetics and phylogenetic relationships of Thelazia species.