Alter fecal microbiota composition and relieve symptom in depression-like rats after treatment by inulin-type fructo-oligosaccharides in Morinda Officinalis
作者单位：State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences University of Macau State Key Laboratory of Quality Research in Chinese Medicine Macau University of Science and Technology Center for Drug Delivery System Shanghai Institute of Materia Medica Chinese Academy of Sciences
会议名称：《2018年第十二届中国药物制剂大会》
会议日期：2018年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the Research Committee of University of Macau (MYRG2016-00090-ICMS-QRCM)
摘 要：Inulin-type fructooligosaccharides（FOS） purified from Morinda officinalis,with degrees of polymerization（DP） from 3 to 9, has been approved as the prescribed oral drug by CFDA in 2012 with the indications for mild and moderate depressive episode, while the bioprocess of this FOS mixtures are largely unknown. This work aimed to illustrate this bioprocess related to gut microbiota and intestine construction. The depression-like rat model was built by chronic unpredictable mild stress（CUMS） procedure for seven weeks, then verified by open field test（OFT） and sucrose preference test（SPT）. Under the three weeks’ treatment period, the fecal sample were collected at day 0, 15 and 22, and further analyzed the microbiota DNA composition using ERIC-PCR method. Hematoxylin and eosin（H＆E） staining was applied to investigate the gut epithelial morphology. Results showed that, rats with depression-like behaviors presented low SPT value（50%, p0.01） and low total travel distance in OFT test（p0.01）. Conversely, SPT value was increased（65%, p0.05） after treatments with FOS, indicating recovering the pleasant sensation. Meanwhile, in partial least square data analysis（PLS-DA） plot, differential modulations of normal and depressive rat model were observed at 0-day microbiota composition. Furthermore, while compared the 22-day plot, there were greatly changed the microbiota composition after treatments（fluoxetine, FOS-DP5 and FOS mixture）. In other side, the colon mucosa, intestinal villus（height and integrity） were severe damaged in intestine of depressive rat model. It was the intestinal inflammatory and severe damage happened in depression-like rats. However, intestinal micro-architecture abnormalities were restored and reversed by FOS treatment. Inferior to FOS, fluoxetine aroused intestinal irritation（cell necrosis, exfoliation and deep staining） on parts of treated rats. On balance, depression-like rat model has significant influence in behavior, intestine structure and micro