Elevated expression of miR-19b enhances CD8+ T cell function by targeting PTEN in HIV infected long term non progressors with sustained viral suppression
作者单位：The First Affiliated Hospital of China Medical University
会议名称：《第十三届全国免疫学学术大会》
会议日期：2018年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
关 键 词：CD8+T cells long-term non-progressors microRNA-19b phosphatase and tensin homolog
摘 要：Human immunodeficiency virus-infected long-term non-progressors(LTNPs) are of particular importance because of their unique disease progression characteristics. Defined by the maintenance of normal CD4+T cells after more than eight years of infection, these LTNPs are heterogeneous. Some long-term non-progressors have ongoing viral production, while others do not and are able to control viral production. The underlying basis for this heterogeneity has not been clearly elucidated. For this study, miRNA expression profiles of LTNPs were assessed and microRNA-19 b(miR-19 b) levels were found to be significantly increased in peripheral blood mononuclear cells of LTNPs with lower rather than higher levels of viral load. We made clear that miR-19 b can regulate the CD8+T cell functions in HIV infection, which has not been addressed before. Overexpression of miR-19 b promoted CD8+T cell proliferation, as well as interferon-γ and Granzyme B expression, while inhibiting CD8+T cells apoptosis induced by T cells receptor stimulation. The target of miR-19 b was found to be the phosphatase and tensin homolog, which regulates CD8+T cells function during HIV infections. Furthermore, we found that miR-19 b can directly inhibit viral production in in-vitro HIV infected T *** results highlight the importance of miR-19 b to control viral levels, which facilitate an understanding of human immunodeficiency virus pathogenesis and provide potential targets for improved immune intervention.