咨询与建议

看过本文的还看了

相关文献

该作者的其他文献

文献详情 >Yu-Ping-Feng inhibits non-smal... 收藏
Yu-Ping-Feng inhibits non-small cell lung cancer tumor growt...

Yu-Ping-Feng inhibits non-small cell lung cancer tumor growth through increasing M1 macrophage polarization and CD4+ T cell cytotoxicity

作     者:Lixin Wang Wenbin Wu Xiaowen Zhu Wanyi Huang Chenyuan Gong Chao Yao Zhongya Ni Shiguo Zhu 

作者单位:Shanghai University of Traditional Chinese Medicine 

会议名称:《第十三届全国免疫学学术大会》

会议日期:2018年

学科分类:1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

关 键 词:Non-small cell lung cancer Yu-Ping-Feng Macrophages CD4+ T cells Tumor microenvironment 

摘      要:Background: The tumor microenvironment(TME) has a deep influence on cancer progression and has become into a new target for cancer treatment. In our previous study, we found that Yu-Ping-Feng(YPF), an ancient Chinese herbal decoction, significantly inhibited the Lewis lung cancer tumor growth in a subcutaneous xenograft tumor model, and prolonged the survival of tumor-bearing mice. But the regulation of YPF on TME is ***: To investigate the inhibitory effect and TME regulation of YPF on the non-small cell lung cancer in an orthotopic lewis lung cancer tumor ***: To access the effect of YPF on non-small cell lung cancer(NSCLC), an orthotopic luciferase stably expressed LLC(LLC-Luc) tumor model was established, and then the survival and the tumor growth were evaluated. To address the TME immune regulation, the percentages of CD4+ T cells, CD8+ T cells, NK cells, regulatory T cells, macrophages, and myeloid-derived suppressor cells in spleens and tumor tissues, the macrophage polarization and CD4+ T cell cytotocixity were analyzed by flow cytometry, biophotonic cell killing activity assay, real-time PCR and ***: YPF significantly prolonged orthotopic lung tumor-bearing mouse survival, and increased the percentages of CD4+ T cell and M1 macrophages and the cytotoxicity of CD4+ T cells. YPF significantly enhanced macrophage-mediated lysis of LLC in a dose-dependent manner, and had no effect on CD4+ T cell-mediated lysis of LLC, but significantly increased CD4+ T cell-mediated lysis after co-incubated with macrophages. In addition, YPF induced M1 macrophage polarization through promoting the phosphorylation of ***: YPF induced M1 macrophages polarization, and then activated CD4+ T lymphocytes, resulting in killing of LLC cells. YPF was a potent regulator of M1 macrophage polarization and might have a promising application in tumor immunotherapy.

读者评论 与其他读者分享你的观点

用户名:未登录
我的评分