Transgenerational memory of gene expression changes induced by heavy metal stress in Rice（Oryza sativa L.）
作者单位：Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics ＆ CytologyNortheast Normal University
会议名称：《中国植物学会第十六次全国会员代表大会暨85周年学术年会》
会议日期：2018年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：Heavy metal stress Transgenerational memory Gene expression DNA methylation
摘 要：Heavy metal toxicity has become a major threat to sustainable crop production ***,considerable interest has been placed on deciphering the mechanisms that allow plants to combat heavy metal *** to deal with heavy metals are largely focused on detoxification,transport and/or *** P1 B subfamily of the Heavy Metal-transporting P-type ATPases（HMAs） were shown to play a crucial role in the uptake and translocation of heavy metals in ***,we report the locusspecific expression changes in the rice HMA genes together with several low copy cellular genes and transposable elements upon heavy metal treatment,and monitored the transgenerational inheritance of the altered expression *** reveal that plants cope with heavy metal stress by making heritable changes in gene expression and further determined gene-specific responses to heavy metal *** found most HMA genes were upregulated in response to heavy metal stress,and furthermore found evidence of transgenerational memory via changes in gene regulation even after removal of heavy *** explore whether DNA methylation was also altered in response to the heavy metal stress,we selected a Tos17 retrotransposon for bisulfite sequencing and studied its methylation state across three *** found the DNA methylation state of Tos17 was altered in response to the heavy metal stress,and showed transgenerational ***,the present study elucidates heritable changes in gene expression and DNA methylation in rice upon exposure to heavy metal stress and discusses implications of this knowledge in breeding for heavy metal tolerance crops.