Nrf2 protects against oxidative stress induced by SiO2 nanoparticles
作者单位：Key Laboratory of Modern Toxicology of ShenzhenShenzhen Center for Disease Control and Prevention State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical SciencesUniversity of Macau
会议名称：《2017环境与公共健康学术会议暨中国环境科学学会环境医学与健康分会、中国毒理学会生化与分子毒理专业委员会2017年年会》
会议日期：2017年
学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学]
关 键 词：Nrf2/ARE signaling pathway silica nanoparticles Nrf2(-/-) mice oxidative stress HO-1
摘 要：Objective:The aim of this study was to investigate the role of nuclear factor erythroid2-related factor 2(Nrf2) in response to oxidative stress exposure to silica nanoparticles(SiO NPs).Methods:The toxicity of SiO NPs-induced oxidative stress,and the activation of Nrf2/ARE signaling pathway were investigated in A549 cells in vitro and mouse model in ***:Exposure to SiO NPs induced significant cytotoxicity in A549 and A549-shNrf2 cells(A549 cells expressing Nrf2 shRNA),and induced activation of the Nrf2/ARE signaling *** Nrf2(-/-)mice,exposure to SiONPs increased the levels of ROS and 8-hydroxy-2’-deoxyguanosine(8-OHdG),and decreased total antioxidant capacity(T-AOC).The disruption of Nrf2/ARE signaling pathway in Nrf2(-/-) mice led to reduced expression of heme oxygenase-1(HO-1) and up-regulation of ***:The Nrf2/ARE pathway might be involved in defending mild to moderate SiO NPs-induced oxidative stress,and Nrf2 could protect against oxidative stress induced by SiONPs.