F-box like motif of CCYV P22 is crucial for silencing suppressor activity by interacting with CsSkp1LB1
作者单位：College of Plant ProtectionHenan Agricultural University
会议名称：《中国植物病理学会第十一届全国会员代表大会暨2018年学术年会》
会议日期：2018年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：Cucurbit chlorotic yellows virus Silencing suppressor Ubiquitin SKP1 F-box Proteomic analysis
摘 要：Plants employ RNA silencing as a virus defense *** response,viruses encode various RNA silencing suppressors to counteract the antiviral ***,we identify P22 as a silencing suppressor of CCYV and show that P22 interacts with Cucurmis sativus orthologs of S-phase kinase related protein 1(SKP1) via its F-box like *** F-box like motif deletion mutant of P22 abolished P22 pathogenicity and silencing suppressor *** analysis of Nicotiana benthamiana leaves expressing P22 and its F-box like motif deletion mutant further confirmed indispensibilty of F-box like motif for the ubiquitin mediated proteolysis and methionine metabolism.S-adenosyl--methionine synthase(SAMS),a key enzyme for HEN1 activity,was undetectable in P22 expressing leaves while expressed in its deletion *** together,our results suggest that P22 acts as silencing suppressor possibly via depriving HEN1 of its substrate S-adenosyl--methionine(SAM) through the ubiquitin mediated proteolysis.