MP3RNA-seq:massive parallel 3' end RNA-seq for highthroughput gene expression profiling
作者单位：State Key Laboratory of Agrobiotechnology and National Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural University
会议名称：《2018中国作物学会学术年会》
会议日期：2018年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：The National Natural Science Foundation of China(31421005 91435206 31701432) the 948 project(2016-X33)
关 键 词：MP3RNA-seq gene expression profiling genotyping QTL mapping
摘 要：Genome-wide gene expression profiling is fundamental for various biological researches *** generation sequencing based RNA-seq is now nearly the routine method of ***,the current RNA-seq approach is not suitable for high throughput experiments due to cost and labor *** we describe a massive parallel 3’ end RNA-seq(MP3 RNA-seq) method,which can generate the sequencing reads of the 3’end of mRNAs for hundreds or thousands of samples in one single *** sequence information resulted from MP3 RNA-seq is sufficient for gene expressional quantification and can also be used for *** further demonstrate its application,we generated the MP3 RNA-seq data for 477 double haploid lines of maize,which were then using for high resolution eQTL and agronomic trait QTL *** summary,MP3 RNA-seq is highly reproducible,accurate,and sensitive for high throughput gene expression profiling,and it can be generally applicable in most of the eukaryotic species.