Tumor-infiltrating Myeloid-derived Suppressor Cells in Patients with Bladder Cancer
作者单位：Shanghai General Hospital
会议名称：《第十三届全国免疫学学术大会》
会议日期：2018年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：MDSCs Bladder cancer Immune environment Immune suppression
摘 要：Background: Both cancer-related inflammation and tumor-induced immune suppression are associated with number of myeloid cell subsets including myeloid-derived suppressor cells(MDSCs). But which kind of myeloid cells play a more important role and the mechanism of tumor promoting is not very *** of Study: For tumor tissues, we tested the proportion of MDCSs in bladder cancer(BC) tissues(n=81) and normal bladder tissues(n=39) by flow cytometry assay. For blood immune environment, we analyzed the proportion of MDCSs of healthy people(n=116) and BC patients(n=226). The biomarker of granulocytic MDSC are CD11 b(violet1), CD45(PEcy7), CD33(PE), CD14-(FITC) and CD15(PE-cy5). The biomarker of monocyte MDSC are CD11 b(violet1), CD45(PE-cy7), CD33(PE), CD14(FITC) and CD15-(PE-cy5). The tumor promoting mechanism was analyzed by gene chip after BC cells and MDSC ***: Our results demonstrated that immune cells from bladder cancer tissues and bladder normal tissues contain two major CD11 b myeloid cell subsets: granulocyte-type and monocyte-type MDSCs. The number of tumor-infiltrating granulocytic but not monocytic myeloid cells in cancer tissues was markedly increased when compared to normal bladder tissues. Granulocyte-type MDSCs can promote progression of BC cells directly, which regulated by many gene ***: We demonstrate that these Granulocyte-type MDSCs is a major kind of immune suppression cells and may contribute to inflammation and immune dysfunction in bladder cancer.