Copy number variations of MTHFSD gene across pig breeds and its association with litter size traits in Chinese indigenous Xiang pig
作者单位：College of Animal Science/Institute of Agro-BioengineeringGuizhou University Tongren University
会议名称：《中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议》
会议日期：2018年
摘 要：Copy number variation(CNV) is a major proportion of genetic variation,which changes the gene structure and dosage and affects gene expression and *** validate the presence and the function of CNV in pig,we used real-time quantitative polymerase chain reaction(qPCR) method to validate a 496 kb CNV region comprising MTHFSD gene on chromosome 6 of Xiang pig detected by single nucleotide polymorphism(SNP) *** we investigated the distribution of the MTHFSD CNV in a total of 545 pigs in four *** 46.2% and 32.7% individuals in the four pig breeds were detected to be types of loss and gain of MTHFSD *** relative copy numbers of MTHFSD gene showed the largest variation range(0-55 copies) in the Xiang pig *** copy numbers of MTHFSD gene presented the positive correlations with the transcript level of MTHFSD gene in adult *** analysis indicated that CNVs of MTHFSD gene was significantly changed the litter size traits of Xiang pigs,and the individuals with CNV gain showed more litter size than the CNV loss *** have reasons to believe that the MTHFSD as RNA-binding protein play an important role in pig reproduction as a result of regulating MTHFS mRNA metabolism.