MicroRNA-29b inhibits silica-induced epithelial-mesenchymal transition and lung fibrosis
作者单位：Department of Occupational Health and Environmental HealthSchool of Public HealthCapital Medical University
会议名称：《2018环境与健康学术会议--精准环境健康：跨学科合作的挑战》
会议日期：2018年
学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学]
关 键 词：silica microRNA-29b lung fibrosis
摘 要：Objectives To elucidate whether microRNA-29 b(miR-29 b) could inhibit silica-induced epithelial-mesenchymal transition(EMT) and lung fibrosis in *** A total of forty C57 BL/6 J mice were randomly divided into four groups:the control,silica,silica + NC,and silica + agomiR-29 b group(n=10).Firstly,the mice were anesthetized with ether and administered with silica suspension *** except for mice in the control group which received the same volume of saline *** 8 th day after silica instillation the mice in the silica + NC and silica + agomiR-29 b groups were injected with negative control or agomiR-29 b at a concentration of25 nmol/mL through tail vein every three *** mice were sacrificed on day 28 after silica *** upper parts of the right lungs were collected for detection of hydroxyproline,and the lower parts of the right lungs were used to examine the gene expression of EMT markers,while the left lungs were fixed in formalin for further pathological ***(1) HE and Masson staining revealed that silica exposure thickened the alveolar septa,increased numbers of cell nodules and areas of collagen fibers in the lung of the *** hydroxyproline assessment presented that silica induced an obvious increase in the lung(p0.05) and immunohistochemistry showed that the protein expression of a-SMA in the silica group increased *** suggested that the mice model of silicosis were successfully established.(2) The injection of agomir-29 b decreased silica-induced lung fibrosis significantly as evidenced by the thickness of the alveolar septum,the decreased number of cell nodules and the area of the blue ***-29 b also decreased the content of hydroxyproline and the protein expression of a-SMA in the lung compared with those in the silica+NC group(p0.05).These results demonstrated that miR-29 b could attenuate silica-induced pulmonary fibrosis in mice.(3) Next the preventive role of miR-29 b on EMT was exam