An asparagine-rich protein Nbnrpl bind with Verticillium dahliae elicitor PevD1 and positively modulate cell death and disease resistance in Nicotiana benthamiana
作者单位:The State Key Laboratory for Biology of Plant Disease and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural Science
会议名称:《2018全国植物生物学大会》
会议日期:2018年
学科分类:09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词:Verticillium dahlia.PevD1.Nbnrpl.protein-protein interaction defense response.disease resistance
摘 要:PevD1 is a fungal protein secreted from Verticillium *** previous research revealed that this proteincould induce hypersensitive responses-like necrosis and systemic acquired resistance(SAR) against tobacco mosaic virus(TMV) and cotton Verticillium *** understand immune activation mechanisms whereby PevD1 elicits defense response,the yeast two hybrid(Y2 H) assay was performed to explore interacting protein of PevDl in Arabidopsis thaliana,and a partner AtNRP(At5 g42050) was *** homolog in Nicotiana benthamiana was identified and designated as *** Nbnrpl could interact with PevD1 via Y2 H and bimolecular fluorescence complementation(BiFC) *** protein mutant assays demonstrated that the DCD domain(residues 200 to 332) of Nbnrpl is required for Nbnrp1-PevD1 interaction through Y2 H and pull-down *** further investigate the roles of Nbnrp1 in PevD1-induced defense response,Nbnrpl-overexpressing lines and Nbnrp1-silence lines were ***1-overexpressing transgenic lines enhanced PevD1-induced necrosis and disease resistance against tobacco mosaic virus(TMV),bacterial pathogen Pseudomonas syringae *** and fungal pathogen *** contrast,Nbnrpl-silence transgenic lines display attenuated the *** is the first report that an asparagine-rich protein Nbnrpl positively regulate PevD1-induced cell death signaling and disease resistance in ***.