Osteogenic differentiation of human adipose-derived mesenchymal stem cells under tensile stress regulated by Let-7i through the Wnt/β-catenin pathway by targeting Wnt10b
作者单位：Oral and Maxillofacial SurgeryStomatological College of Nanjing Medical University General Department Stomatological College of Nanjing Medical University
会议名称：《第十四次中国口腔颌面外科学术会议》
会议日期：2018年
学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学]
关 键 词：hASCs osteogenic differentiation tensile stress Wnt/β-catenin Let-7i Wnt10b
摘 要：Objective:Bone tissue engineering provides new methods to repair bone defects in oral and maxillofacial region, and human adipose-derived stem cells(hASCs) as seed cells is the key point. Tensile stress can promote the osteogenic differentiation of hASCs in vitro. Methods:Microarray analysis data showed that Let-7 i was markedly downregulated in h ASCs under tensile stress treatment. Bioinformatic analysis and luciferase reporter assays revealed that Wnt10 b was a direct target of Let-7 i. Results:Under tensile stress, mimics-mediated gain-function studies revealed that forced expression of Let-7 i inhibited osteogenic differentiation of hASCs, whereas inhibition of Let-7 i by inhibitor obviously promoted osteogenic differentiation. Furthermore, Let-7 i overexpression decreased activity of the Wnt/β-catenin pathway, as indicated by lower expression of Wnt10 b, active β-catenin in Let-7 i-treated hASCs. By contrast, Let-7 i inhibition activated the Wnt/β-catenin pathway. Conclusion:Taken together, these results demonstrate that, under tensile stress, Let-7 i negatively regulates hASCs osteogenic differentiation through the Wnt/β-catenin pathway by directly targeting Wnt10 b.