MicroRNA-29b inhibits supernatants from silica treated macrophages from inducing extracellular matrix synthesis in lung fibroblasts
作者单位：School of Public HealthCapital Medical University
会议名称：《2018环境与健康学术会议--精准环境健康：跨学科合作的挑战》
会议日期：2018年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100402[医学-劳动卫生与环境卫生学] 10[医学]
基 金：National Natural Science Foundation Youth Project(81602832)
关 键 词：miR-29b silica fibroblast
摘 要：Objectives Silicosis is pathologically characterized by diffused pulmonary fibrosis and abundant deposition of extracellular matrix(ECM) *** ECM is mainly secreted by myofibroblasts which are the activated state of ***-29 b(miR-29 b) is one of the well-known microRNAs involved in fibrosis,but its roles in silicosis have not been *** In this study,we hypothesized that miR-29 b might play a protective role in the progression of *** assay,qRT-PCR,immunofluorescence and western blotting were *** The results demonstrated that the supernatants from silica-treated macrophages not only caused the proliferation of fibroblasts(NIH-3 T3 and MRC-5)but were also involved in the downregulation of miR-29 *** they could induce fibroblast activation,increasing the expression of ECM components such as collagen 1 and collagen3,in a silica dose-dependent ***,overexpression of miR-29 b by transfecting mimics markedly reduced the expression of ECM components and inhibited ECM *** These findings indicate that miR-29 b inhibits the supematants from silica treated macrophages from inducing extracellular matrix synthesis,thus miR-29 b might have a strong anti-fibrotic capacity in silicosis and serve as a potential therapeutic agent for the treatment.