Cardioprotective effects of Salvia miltiorrhiza Bunge and Lignum dalbergiae odoriferae on rat myocardial ischemia/reperfusion injury
作者单位：Department of Pharmacy Xijing Hospital Fourth Military Medical University
会议名称：《2015年中国药学大会暨第十五届中国药师周》
会议日期：2015年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：myocardial ischemia/reperfusion injury Salvia miltiorrhiza Bunge Lignum dalbergiae odoriferae the maximum and minimum effective points anti-oxidant anti-inflammatory
摘 要：Aim: Salvia miltiorrhiza Bunge（SM） and lignum dalbergiae odoriferae（DO） are both traditional Chinese medicine that have cardioprotective effects. Here, we further examined the combined effects of SM and DO on rat myocardial ischemia/reperfusion injury. The possible mechanism of SM and DO also were elucidated. Material and Methods: DO was divided into aqueous extract of lignum dalbergiae odoriferae（DOW） and lignum dalbergiae odoriferae oil（DOO）. Sprague-Dawley rats were randomized to seven groups: sham group, model group, treatment groups including SM（10 g/kg）, DOW（5 g/kg）, DOO（0.5 ml/kg）, SM+DOW（10 g/kg+5 g/kg）, SM+DOO（10 g/kg+0.5 ml/kg）. Rats were pretreated with homologous drug for 7 days and then subjected to 30 min of ischemia followed by 180 min of reperfusion. Electrocardiogram（ECG） and heart rate were monitored and recorded continuously. At the end of reperfusion, blood samples were collected to determine the serum levels of creatine kinase-MB（CK-MB） and lactate dehydrogenase（LDH）. Hearts were harvested to assess heart-body rate, infarct size and histopathological changes as well. Maximum and minimum effective points were determined by measuring indicators associate with myocardial injury at different time-points of reperfusion（5 min, 15 min, 30 min, 45 min, 60 min, 120 min, 180 min）. The potential therapeutic mechanism of SM and SM+DOO were carried out by detecting superoxide dismutase（SOD）,malondialdehyde（MDA）, tumor necrosis factor-alpha（TNF-alpha） and interleukin 6（IL-6）. Results: The results showed SM and DO can ameliorate cardiac function respectively, and this cardioprotective effect was further strengthened by their combinations. Among all the combinations, SM+DOO showed predominant potential to improve ECG and heart rate, reduce heart-body rate（28.5% ±1.4%, P0.01 vs model）and myocardial infarct size（20.96%±1.61%,P0.01 vs model, P0.05 vs SM）, attenuate histopathological damage, decrease the levels of CK-MB and LDH（P0.01 vs model, P0.05 vs SM）. Th