A high-resolution genetic map and fine QTLs mapping of seed size and oil content traits in castor bean
作者单位：Key Laboratory of Economic Plants and BiotechnologyYunnan Key Laboratory forWild Plant ResourcesKunming Institute of BotanyChinese Academy of Sciences University of the Chinese Academy of Sciences Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of ChinaMinistry of EducationSouthwest Forestry University
会议名称：《2018全国植物生物学大会》
会议日期：2018年
关 键 词：Castor bean genetic map pseudo-chromosome QTL seed yield oil content
摘 要：Castor bean(Ricinus communis L.,Euphorbiaceae) is a critical biodiesel crop and its seedderivatives have industrial *** to the lack of any genetic map the breeding and genetic improvement of castor bean has been largely *** the rapid development of sequencing technologies,genotyping-by-sequencing(GBS) has became one of the most popular methods for developing genomic SNP *** GBS for 200 individuals within the recombinant inbred line(RIL) population generated8,896 SNP *** on the available draft reference genome(at the scaffold level),we obtained a high-density genetic map with 10 linkage groups(LGs),spanning1,852.33 cM with an average distance of about 0.43 ***,996 scaffolds from draft reference genome with 296.01 Mb,accounting for 84.43%of the whole genome,were anchored into 10 *** on the assembled castor bean genome we performed a genome synteny analysis within the castor bean genome and between castor bean and *** results showed new evidence that the castor bean genome might have undergone paleopolyploidy events during *** explored the evolutionary divergence of castor bean from other members of Euphorbiaceae,such as physic nut,cassava and rubber *** on the RIL population and genome resequencing,we identified 16 QTLs that control seed yield(covering 851 candidate genes) and 2 QTLs that control seed oil content(covering 300 candidate genes).This study is the first to assemble a pseudo-chromosome scale genome of castor bean based on a high-density genetic map,providing new evidence towards understanding gene and genome variation during the evolution of castor *** and candidate genes were identified that potentially control seed yield and oil *** provides a useful gene resource for molecular breeding and genetic engineering in castor bean.