Resolving the α-Glycosidic Linkage of Arginine-Rhamnosylated Translation Elongation Factor P(EF-P) Triggers Generation of the First Arg Rha Specific Antibody

作     者：Xiang Li Ralph Krafczyk Jakub Maco?ek Yu-Lei Li Yan Zou Bernd Simon Xing Pan Qiu-Ye Wu Fang Yan Shan Li Janosch Hennig Kirsten Jung Jürgen Lassak 胡宏岗 

作者单位：Department of Organic Chemistry School of Pharmacy Second Military Medical University School of Pharmacy Wei Fang Medical University 

会议名称：《第二届糖合成化学青年学术研讨会》

会议日期：2017年

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

摘      要：A previously discovered posttranslational modification strategy–arginine rhamnosylation–is essential for EF-P dependent rescue of polyproline stalled ribosomes in clinical relevant species such as Pseudomonas aeruginosa and Neisseria meningitidis2].However,almost nothing is known about this new type of N-linked glycosylation.In the present study,we used NMR spectroscopy to show for the first time that theaanomer of rhamnose is attached to Arg32 of EF-P,demonstrating that