Separation of a minor lignan from Sinopodophyllum emodi using HSCCC combined MPLC guided by HPLC/MS
作者单位：Key Laboratory of Tibetan medicine Research Northwest Institute of Plateau Biology Chinese Academy of Sciences
会议名称：《中国化学会第十届全国天然有机化学学术会议》
会议日期：2014年
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 070303[理学-有机化学] 0703[理学-化学]
摘 要：正An efficient method using high performance liquid chromatography coupled with mass spectrometry（HPLC/MS）,medium pressure liquid chromatography（MPLC）and high-speed counter-current chromatography（HSCCC）was successfully established for rapid screening,identification,enrichment and separation of a minor lignan 4’-demethyldeoxypodophyllotoxin from Sinopodophyllum *** this work,the crude extract was