Emergence of a Novel Bluetongue Virus Serotype in Xinjiang, China
作者单位：Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences
会议名称：《第十届全国免疫学学术大会》
会议日期：2015年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：Bluetongue virus Epidemiology New serotype
摘 要：Background:Bluetongue virus(BTV)belongs to the genus Orbivirus and infects ruminants,camelids and occasionally large carnivores.The recent identification of BTV-25,BTV-26,and BTV-27 in Switzerland,Kuwait and France,respectively,suggests that additional BTV serotypes may circulate around the world.Objective:Through conventional epidemiological investigations confirmed epidemiological situation of BTV in Xinjiang,China.Methods:Conventional methods were used to isolate sera for BTV antibody detection and RNA from red cells for BTV antigen detection.Erythrocyte preparations from BTV antigen-positive samples were inoculated into chicken embryo veins and then passaged three times in BHK-21 cells to isolate virus.Full-length c DNA copies of seg-2 from XJ1407were synthesized and sequenced as previously described(Maan,et al.,2007).Results:One hundred and twenty-six blood samples were collected from healthy sheep and goats in Xinjiang,China,during July of 2014.Seventy-three samples(57.93%)were BTV VP7 antibody-positive and 39 samples(30.95%)were BTV antigen-positive.BTV strain XJ1407 was isolated from the blood of BTV antigen-positive goats.Virus neutralization test(VNT)results showed that XJ1407 strain was not neutralized by BTV14 reactive antisera.Neutralization tests for XJ1407 using BTV serotype 257 positive sera were not assessed due to the lack of characterized antisera and control viruses in China.Sequence analysis of seg-2 of XJ1407 showed 75%nucleotide identity and 78%amino acid identity with BTV27 isolate obtained in 2014.Conclusions:The results of VNT and sequence analysis suggest XJ1407 may be a novel BTV serotype.