Study of N7-（2-carbamoyl-2-hydroxyethyl）Guanine in Urine Samples Repeatedly Collected from Acrylamide-Exposed Workers
作者单位：Institute of Environmental and Occupational Health SciencesNational Yang Ming University Department of Food ScienceNational Pingtung University of Science and Technology Division of Environmental Health and Occupational MedicineNational Health Research Institutes School of Chinese MedicineChina Medical University Institute of Occupational Medicine and Industrial HygieneNational Taiwan University
会议名称：《第八届海峡两岸毒理学研讨会》
会议日期：2015年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100402[医学-劳动卫生与环境卫生学] 10[医学]
关 键 词：Acrylamide N7-(2-carbamoyl-2-hydroxyethyl)guanine glycidamide elimination kinetics
摘 要：Acrylamide（AA）is a widely-used industrial chemical,present in tobacco smoke and high-temperature processed foods,and classified as a probable human *** activation from AA to glycidamide（GA）to form DNA adducts is critical to AA carcinogenicity.N7-（2-carbamoyl-2-hydroxyethyl）guanine（N7-GAG）,the most abundant DNA adduct induced by GA,was recently detected in urine of smokers and *** study aims to analyze N7-GAG in urine samples repeatedly collected from AA-exposed workers to explore its elimination ***,8 exposed and 36 control workers were recruited for this ***-and post-shift urine samples were collected from the exposed group in parallel with personal sampling for 8 consecutive days and from the control group for one day.N7-GAG was analyzed with our newly-developed two-step SPEs and on-line SPE liquid chromatography-electronspray ionization/tandem mass spectrometry（LC-ESI-MS/MS）***-and GA-mercapturic acids（AAMA and GAMA）were also analyzed to serve as biomarkers for AA current *** exposed workers excreted significantly higher N7-GAG levels than the controls.N7-GAG was significantly associated with AAMA and GAMA,*** of the N7-GAG data with linear mixed-effect model reveals a mean half-life at 346.5 hrs with a 95%confidence interval（140.3-737.2）*** from this study suggest that urinary N7-GAG levels could be elevated by occupational exposures to AA and intra-and inter-individual differences were damped with the half-life of the biomarkers.