MiR-133b-5p regulates the expression of the heat shock protein 70 during rat neuronal cell apoptosis induced by the gp120 V3 loop peptide
作者单位：Department of PharmacyThe Third Affiliated Hospital of Guangzhou Medical University Department of Dermatology and RheumatologyFoshan Maternity & Child Heath Care Hospital Department of Reproductive Medicine CentreThe Third Affiliated Hospital of Guangzhou Medical University Guangdong Provincial Key Laboratory of Reproductive MedicineGuangzhou Medical University at Guangzhou
会议名称：《2017年广东省药师周大会》
会议日期：2017年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 100205[医学-精神病与精神卫生学] 10[医学]
基 金：supported by the Science & Technology Foundation of Guangdong Province (No.2013B021800188) to Xia C Open Fund of Guangdong Provincial Key Laboratory of Reproductive Medicine (No.2012125) to Xiao G Guangdong Family Planning and Reproductive Health Funding(No.20133064) to Yang J
关 键 词：miR-133b-5p gp120 microRNA heat shock protein 70 apoptosis
摘 要：Background:Neuronal cell dysfunction and apoptosis, the main causes of HIV-associated dementia, and its underlying mechanism are important unsolved health problems. Many research reports suggest that miRNAs regulate HIV-1-induced apoptosis. Methods:We used the HIV-1 gp120 V3 Loop peptide to induce primary rat cortical neurons apoptosis. Next, we used a microRNA microarray to identify the significant changes of miRNA in the rat cortical neurons treated with the gp120 V3 loop peptide. We used western blot and real-time PCR to measure the regulation of heat shock protein 70 by ***:In response to the gp120 V3 loop peptide treatment, rat cortical neurons exhibited 11 up-regulated and 21 down-regulated miRNAs. We further examined miR-133b-5p, a microRNA that was up-regulated more than 118-fold. In addition, both HSP70 mRNA and protein expression were dose-dependent in rats cortical neurons treated with gp120 V3 loop peptide for 48 hours. Conclusion:MiR-133b-5p could regulate heat shock protein 70(HSP70) at both transcription and translation levels. Rno-miR-133b-5p might be less significant for the gp120 V3 loop peptide induced neuron apoptosis. Thus, we discovered a potential new target for the regulation of HIV-1 gp120-induced apoptosis.