Identification of γδ T cells from zebrafish provides insights into their important role in adaptive humoral immunity
作者单位：College of Life Scienceszhejiang university Key Laboratory for Cell and Gene Engineering of Zhejiang Province Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and Technology
会议名称：《第十二届全国免疫学学术大会》
会议日期：2017年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：γδ T cells adaptive humoral immunity zebrafish
摘 要：The γδ T subset is an evolutionarily primitive T cell population characterized by distinct TCRs and functional roles in the innate and adaptive *** has been well documented in humans and mouse ***,little is known about the occurrence and existence of this T cell subset in ancient *** the present study,we report the molecular and cellular characterizations of γδ T cells from a zebrafish(Danio rerio) *** constant regions of zebrafish TCR-γ(DrTRGC) and TCR-8(DrTRDC) were initially *** γδ T cells accounted for 7.7% to 20.5%of the total lymphocytes in spleen,kidney,peripheral blood,skin,gill and intestine *** possess typical morphological features of lymphocytes with a surface phenotype of γδ*** γδ T cells functionally showed a potent phagocytic ability to both soluble and particulate *** can act as an antigen presenting cell(APC)to initiate the antigen(KLH)-specific CD4 T cell activation and to induce the subsequent B cell proliferation and antibody(IgM) ***,zebrafish γδ T cells also play a critical role in fish specific IgZ production in intestinal *** findings indicated that γδ T cells had been originated as early as teleost fish,which providing valuable insights into the evolutionary history of T cell *** hope that this study would be used as a guide to develop a zebrafish model for the cross-species investigation of γδ T cell biology.