DNA Amplification in Streptomyces hygroscopicus 10-22
作者单位:The State Key Laboratory of Microbial TechnologyShandong University Shandong Academy of Medical Sciences School of Municipal and Environmental EngineeringShandong Jianzhu University
会议名称:《第四届泛环渤海生物化学与分子生物学会学术交流会》
会议日期:2013年
学科分类:0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
摘 要:T110,obtained through mutational cloning of Streptomyces hygroscopicus 10-22 with pIJ702 derivatives containing 10-22 genomic DNA fragment(1-9kb in size),was found to contain amplified DNA sequence with c.300 copies per chromosome by running the total DNA digestions in both conventional gel electrophoresis