Hugan Qingzhi exerts anti-inflammatory effects in a rat model of non-alcoholic fatty liver disease
作者单位：Guangdong Province Key Laboratory for Biotechnology Drug CandidatesSchool of Biosciences and BiopharmaceuticsGuangdong Pharmaceutical University Department of PharmacyZhujiang HospitalSouthern Medical University Department of PharmacyYue Bei people's HospitalShantou University Pharmaceutical Research & DevelopmentZhujiang HospitalSouthern Medical University
会议名称：《2016年广东省药师周大会》
会议日期：2016年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1001[医学-基础医学(可授医学、理学学位)] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by grants from the Nature Science Foundation of China(No.81274160) the Natural Science and Technology Development Project of Guangdong Province(No.S2012010009380)
关 键 词：Non-alcoholic fatty liver disease Inflammation Hugan Qingzhi tablet Silent information regulator 1 Nuclear factor-κB
摘 要：Ethnopharmacological relevance:The Hugan Qingzhi tablet(HQT) is a traditional Chinese medicine used for treating NAFLD(non-alcoholic fatty liver disease).The present study evaluated the anti-inflammatory effects of HQT in rats with *** and methods:NAFLD was induced by a high-fat diet over a 12-week period,during which HQT was administered daily to the experimental *** markers,histopathological data,and oxidative stress/antioxidant biomarkers were ***-inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor α(TNF-α) and interleukin-6(IL-6) were detected by enzyme-linked *** of silent information regulator 1(SIRT1) and acetylated-Nuclear-factor kappa B-p65(Ac-NF-κB-p65) were performed by western ***:At high and moderate doses,HQT was highly effective in decreasing serum alanine aminotransferase(p0.01),aspartate aminotransferase(p0.01),hepatic total cholesterol(p0.01),triglycerides(p0.01),and free fatty acid levels(p0.01).This was associated with reduced histological steatosis and inflammation,and significantly increased superoxide dismutase,glutathione peroxidase(GPX),and total antioxidant serum activities(p0.01),contrasting with a significant decrease in hepatic malondialdehyde levels(p0.01).Moreover,high and moderate doses of HQT reduced hepatic levels of the pro-inflammatory cytokines TNF-α(p0.01),IL-1β(p0.01),and IL-6(p0.01),enhanced SIRT1 expression and depressed Ac-NF-κB-p65 expression at protein ***:In our NAFLD rat model,HQT exerted substantial anti-inflammatory and antioxidant activities,possibly involving the regulation of SIRT1 and Ac-NF-κB-p65 expression.