Neuroprotection of ceruloplasmin on experimental stroke model
作者单位：State Key Laboratory of Biotherapy West China Hospital Sichuan University
会议名称：《中国神经科学学会第十二届全国学术会议》
会议日期：2017年
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学]
关 键 词：Ischemic Stroke ceruloplasmin MCAO Neuroprotection
摘 要：Background: Cerebrovascular disease(CVD) is one of the three illnesses which cause most deaths all over the world. In cerebrovascular diseases, ischemic stroke has the highest incidence. The current clinical treatment program for ischemic stroke cannot fundamentally handle high mortality and high morbidity after the onset of ischemic stroke, so it’s urgent that we need a more effective treatment to cure ischemic stroke. Ceruloplasmin(Cp) can oxidize Fe to Fe, and preventing Fe to cause damage therefore may be able to rescue ischemia/reperfusion ***: We established middle cerebral artery occlusion(MCAO) mice model, divided MCAO mice into three groups randomly, and injected with saline, ceruloplasmin and Apo-ceruloplasmin(Apo-Cp) respectively. The neurological function of MCAO model mice was evaluated by Bederson score method. The movement coordination ability was evaluated by rotarod test. The hippocampal Cp was detected by western blot and the activity was detected by Fe acquisition and Fe loss assays. The area of cerebral infarction was observed by TTC staining at 24 hours after ischemia-reperfusion. Results: The neurological function of MCAO mice treated with Cp was significantly improved compared with the mice with saline. Cp expression in ischemic site of MCAO mice was significantly increased compared with non-ischemic site, but the activity was unchanged. The TTC staining showed that the cerebral infarction area of MCAO model mice treated with Cp was significantly lower than the saline group. MCAO mice treated with Apo-Cp showed no improvement. Conclusions: Ceruloplasmin can rescue the ischemia/reperfusion injury, which should be explored further as drug candidate.