旋覆花来源的IVSE抑制巨噬细胞RAW264.7 NO生成的作用及分子机理(英文)
作者单位：天津医科大学药学院
会议名称：《全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议》
会议日期：2015年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：国家自然科学基金资助项目(81373441 81202542)
摘 要：Our previous study showed that the extract of Inula japonica Thunb(***)has anti-inflammatory ***,weisolated1,6-dihydroxy-4 H-1,10-secoeudesma-5(10),11(13)-dien-12,8-olide(SE),6-isobutyryloxy-1-hydroxy-4 H-1,10-secoeudesma-5(10),11(13)-dien-12,8-olide(IBSE),and6-isovaleryloxy-1-hydroxy-4 H-1,10-secoeudesma-5(10),11(13)-dien-12,8-olide(IVSE)from ***,and investigated their inhibitory effects on nitric oxide(NO)production in LPS-stimulated RAW264.7 *** them,IVSE displayed the most potent inhibition of NO *** we investigated the potential anti-inflammatory *** dramatically decreased the expression of inducible nitric oxide synthase(iNOS),reduced the translocation of the Nuclear factor B(NF-B)subunit p65 into the nucleus by interrupting the phosphorylation and degradation of inhibitor kappa B(I B)-,and inhibited the activation of the upstream mediator IkB kinase(IKK).Furthermore,our results indicated that IVSE inhibited the phoshorylation of mitogen-activated protein kinases(MAPKs)including extracellular regulated kinases(ERK1/2),c-Jun N-terminal kinases(JNK)and *** together,our results showed that IVSE exhibited anti-inflammatory activity by inhibiting NO production,in which inactivation of NF-B and MAPKs might be involved.