A single amino acid determines the catalytic efficiency of two alkenal double bond reductases produced by the liverwort Plagiochasma appendiculatum
作者单位：Key Laboratory of Chemical Biology of Natural Products Ministry of Education School of Pharmaceutical Sciences Shandong University
会议名称：《中国化学会第十届全国天然有机化学学术会议》
会议日期：2014年
学科分类：081705[工学-工业催化] 08[工学] 0817[工学-化学工程与技术]
基 金：the support of the National Natural Science Foundation of China(Nos.31170280 and 31370330)
摘 要：正Alkenal double bond reductases（DBRs）catalyze the NADPH-dependent reduction of theα,β-unsaturated double bond of many secondary *** alkenal double bond reductase genes Pa DBR1 and Pa DBR2 were isolated from the liverwort species Plagiochasma *** Pa DBR2 protein had a higher catalytic activity than Pa DBR1 with