Screening and Analysis of Effector Proteins from Puccinia triticina
作者单位：Department of Plant PathologyAgricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei ProvinceNational Engineering Research Center for Agriculture in Northern Mountainous Areas
会议名称：《中国植物病理学会2017年学术年会》
会议日期：2017年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Puccinia triticina Effector proteins Screening
摘 要：Leaf rust caused by the fungus Puccinia triticina（Pt） is a major constraint to wheat production *** molecular events that underlie Pt pathogenicity are largely *** all rusts,Pt forms a specialized infection structure called haustoria to take nutrients and water from host plant tissue,and to secrete pathogenicity factors called effector *** this study,we extracted the total RNA of urediniospores,germinated urediniospores and infected wheat leaves harvest at 6 d from three different virulence strains of Pt and used Illumina Hiseq sequencing platforms to assemble the transcriptomes and then we got 46008 *** effectors were screened through Signal P 4.1,Target P 1.1,TMHMM 2.0 and Effector P,and 325 genes which constitute candidate effectors were ***-PCR analysis confirmed the expression patterns of 96 candidate effectors and 42 genes showed aspecial increase in various stages which may be involved in the pathogenic process.33 candidate effectors were cloned successfully and 17 were identified to be able to suppress the cell death caused by BAX in tobacco *** analysis of candidate effectors is an initial step towards functional research and established the foundation forclarify the pathogenic mechanism of leaf rust pathogens.