Thymoquinone attenuates monocrotaline-induced pulmonary arteryhypertension via inhibiting pulmonary arterial remodelingin rats
会议名称：《2016年浙江省医学会心电生理与起搏学术年会》
会议日期：2016年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：pulmonary arterial hypertension thymoquinone proliferation migration p38MAPK/NF-κB
摘 要：Background:Pulmonary artery remodeling induced by excessproliferation,migration and apoptosis resistance of pulmonary arterial smooth muscle cells(PASMCs)is akey component in pulmonary artery hypertension(PAH).Thymoquinone(TQ) triggers cancer cells apoptosis through *** addition,TQ inhibits migration of human nonsmall-cell lung cancer cells and human glioblastoma ***:In the current study,we investigated effects of TQ on MCT-induced PAH in rats and its underlying ***:After 2 weeks of monocrotaline injection(MCT,60 mg/kg),Male Sprague-Dawley rats received TQ(8 mg/kg,12 mg/kg,16 mg/kg) or olive oil per day for 2 *** changes,right ventricular hypertrophy,and lung morphologicalfeatures were examined 4 weeks *** addition,TUNEL,PCNA,a-SMA,Bax and Bcl-2 were detected by Immunohistochemistry ***,Bcl-2,cleaved caspase-3,cleaved poly(ADP-ribose)polymerase(PARP) MMP2,MMP9 and activation of p38 MAPK and NF-κB wereassessed by Western ***:MCT-induced an increase in pulmonary blood pressure and right ventricular hypertrophy,which were attenuated by TQ *** also blocked MCT-inducedpulmonary arterial remodeling,proliferation ofPASMCs,elevation of MMP2 and downregulation of ratio of Bax/Bcl-2,cleavedcaspase-3 and ***,TQ inhibited MCT-induced activation of p38 MAPK and NF-κ***:TQ ameliorates MCT-induced pulmonary artery hypertension by inhibiting pulmonary arterial remodeling partially via p38 MAPK/NF-κB signalingpathway in rats.