Function of the Ss-SFH1 Transcription Factor in Sclerotinia sclerotiorum
作者单位：College of Plant SciencesJilin University
会议名称：《中国植物病理学会2017年学术年会》
会议日期：2017年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
基 金：financially supported by the National Natural Science Foundation of China(31271991 31471730)
摘 要：Sclerotinia sclerotiorum（Lib.） de Bary is a necrotrophic plant pathogen with a worldwide ***,a gene named Ss-SfhI was cloned in ***,which encodes a snf5-box-containing *** predicted Ss-SFH1 protein also has a GATA Zn-finger domain in a C-terminal *** investigate the role of Ss-SFHl in ***,the partial sequence of Ss-SFH1 was cloned and RNA interference（RNAi）-based gene silencing method was employed to alter the expression of *** silenced mutants Ss-SFHl RNA levels reduced exhibited slow hyphal *** and oxalic acid accumulation were reduced in Ss-Sfh1 RNAi *** assays demonstrated that pathogenicity in RNAi-silenced strains was significantly compromised with the development of a smaller infection lesion on soybean *** addition,Ss-Sfh1-silenced transformants were more tolerant to oxidative stress compared with the wild-type ***,the expression levels of putative probably involved in ROS production-related genes were significantly different in silenced *** the results suggest that Ss-SFH1 is involved in hyphal growth,virulence and the tolerance to oxidative stress in ***.
