Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model in Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro
作者单位：Clinical Medical CollegeBeijing University of Chinese Medicine State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical Sciences University of Macau Department of Pharmaceutical PreparationXiyuan HospitalChina Academy of Chinese Medical Sciences
会议名称：《第八次全国中西医结合中青年学术论坛》
会议日期：2016年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1001[医学-基础医学(可授医学、理学学位)] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by the Science and Technology Development Fund of Macao SAR(No.078/2011/A3 and 134/2014/A3) Research Committee of University of Macau(No.MYRG2015-00182-ICMS-QRCM) Beijing Municipal Science and Technology Commission(No.Z141100002214011),China
关 键 词：angiogenesis paeoniflorin zebrafish. human umbilical vein endothelial cell
摘 要：Objective:To investigate the pro-angiogenic effects of paeoniflorin(PF)in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells(HUVECs).Methods:In vivo,the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1:EGFP)y1 transgenic *** 24 h post fertilization(hpf) embryos were pretreated with vascular endothelial growth factor(VEGF) receptor tyrosine kinase inhibitor II(VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 *** formation of intersegmental vessels(ISVs) was observed with a fluorescence *** mRNA expression of fins-like tyrosine kinase-1(flt-1),kinase insert domain receptor(kdr),kinase insert domain receptor like(fdrl) and von Willebrand factor(vWF) were analyzed by real-time polymerase chain reaction(PCR).In vitro,the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation,migration and tube formation were ***:PF(6.25- 100μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF(25 to 100μmol/L),thereby restoring the mRNA expressions of flt-1,Mr,kdr1 and vWF,which were down-regulated by VRI *** addition,PF(0.001- 0.03 μmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0-10 umol/L and tube formation at 0.3 umol/***:PF could promote angiogenesis in zebrafish in vivo and in HUVECs in vitro.