Fluorescence in situ hybridization rapidly detects three different pathogenic bacteria in urinary tract infection samples
作者单位：Department of Clinical Laboratory Renmin Hospital of Wuhan University
会议名称：《第二届中国临床微生物学大会暨微生物学与免疫学三江论坛》
会议日期：2011年
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 1010[医学-医学技术（可授医学、理学学位）] 10[医学]
关 键 词：Rapid detection Bacteria Fluorescence in situ hybridization Urinary tract infection
摘 要：Objective The detection of pathogenic bacteria in urine is an important criterion for diagnosing urinary tract infections（UTIs）.By using fluorescence in situ hybridization（FISH） with rRNA-targeted,fluorescently labeled oligonucleotide probes,bacterial pathogens present in urine samples were identified within 3-4 *** this study,three probes that are specific for Escherichia coli,Enterococcus faecalis and Staphylococcus aureus were designed based on the conserved 16 SRNA sequences,whereas probe Eub338 broadly recognizes all *** and Discussion The samples for these experiments included 76 *** infected samples,32 *** infected samples and 9 *** infected *** to conventional methods of bacterial identification,the FISH method produced positive results for 90%of the samples *** microscopy（magnification,×100） of microorganisms from urine samples after *** A:FISH with probes specific for Escherichia coli（green signal）;Part B:FISH with probes specific for Enterococcus faecalli（red signal）;Part C:FISH with probes specific for Staphylococcus aureus（green signal）.Conclusions FISH has the potential to become an extremely useful diagnostic tool for UTIs because it has a quick turnaround time and high *** This work has been supported by the Fundamental Research Funds for the Central Universities