Application of Crude Protease from Cheap and Local Raw Material as a Biopesticide for the Disruption of Pomacea Canaliculata Eggs
作者单位：School of Bioprocess EngineeringUniversity Malaysia Perlis
会议日期：2015年
学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护]
基 金：supported in part by the Malaysian Ministry of Science Technology and Innovation(MOSTI)under Science Fund Grant(9005-00056)
关 键 词：Aspergillus oryzae pomacea canaliculata hatchability eggs
摘 要：The application of crude protease of Aspergillus oryzae to suppress the hatchability of Pomacea canaliculata eggs is highlighted. The enzyme was produced through solid state fermentation(SSF) using cassava, which is cheap and abundantly available. It was found that the enzyme suppressed the hatchability of P. canaliculata eggs as much as 30% and 20% at 20.8U/ml enzyme for 3 and 9 days old eggs respectively. The ANOVA result revealed that the hatchability was significantly affected by both protease concentration as well as egg’s age. The LC for 3 and 9 days old eggs were 3.29 U/ml and 3.54 U/ml, respectively;while LC for 3 and 9 days old eggs were 19.11 U/ml and 20.09 U/ml respectively. It is proposed that the crude protease propagated from SSF using locally available and cheap material should be considered as an alternative biopesticide to suppress the hatchability of P. canaliculata eggs.