Differential regulation of clathrin and its adaptor proteins, AP-2 and the TPLATE complex, during their membrane recruitment in Arabidopsis
作者单位：College of Chemistry and Life SciencesZhejiang Normal University Institute of Genetics and Developmental BiologyChinese Academy of Sciences Department of Biochemistry and Molecular BiologyPennsylvania State UniversityUniversity Park Department of BiologyInstitute of Agricultural SciencesETH Zurich Department of Plant Systems BiologyVIBDepartment of Plant Biotechnology and BioinformaticsGhent University College of Biological Sciences and BiotechnologyBeijing Forestry University
会议名称：《2016年全国植物生物学大会》
会议日期：2016年
学科分类：09[农学] 0903[农学-农业资源与环境]
关 键 词：Adaptor Protein 2 auxin clathrin endocytosis salicylic acid TPLATE complex tyrphostin A23 Arabidopsis
摘 要：In plants,clathrin-mediated endocytosis(CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes,Adaptor Protein 2(AP-2) and the TPLATE complex(TPC),and is negatively regulated by the hormones auxin and salicylic acid(SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane(PM) to regulate CME are however poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23(Tyr A23) reduce the membrane association of clathrin and AP-2,but not that of the TPC,whereas auxin solely affected clathrin membrane association,in Arabidopsis thaliana. Genetic and pharmacological experiments revealed that loss of AP2μ or AP2σ partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2σ,but not AP2μ,was required for SA-and Tyr A23-dependent inhibition of CME. Furthermore,we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells,the TPC is necessary for clathrin PM association in AP-2 deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.