Piwill Mediates Meiosis during Spermatogenesis in Chicken
作者单位：Jiangsu Key Laboratory for Animal GeneticBreeding and Molecular DesignYangzhou University
会议名称：《第七届中国畜牧科技论坛》
会议日期：2016年
关 键 词：chicken Piwill meiosis spermatogenesis germ cells
摘 要：Piwil1 mediates spermatogenesis and ensures stable cell division rates in germline cells in ***,the involvement of Pizvil1 in poultry spermatogenesis and meiosis is poorly *** the present study,we used TaqMan RT-qPCR to characterize Piwil1 mRNA expression in different types of spermatogenic cells,including primordial germ cells(PGCs),spermatogonial stem cells(SSCs),spermatogonia cells(Sa),tetraploid cells(Tp),round sperm cells(Rs),mature sperm,and in PGCs treated with retinoic *** results revealed that Piwil1 is differentially expressed during spermatogenesis in *** to PGCs,SSCs,Tp,and Sa,Rs cells presented the highest Piwil1 mRNA expression *** acidsignificantly upregulated Piwil1 and Stra8 mRNA expression as well as Piwil1 for protein levels in chicken *** addition,retinoic acid induced PGCs to progress through all the meiotic stages,eventually leading to haploid cell formation,which was determined using flow cytometry and western blot *** together,our results showed that during spermatogenesis,Piwil1 was first expressed at low levels in germ stem cells,PGCs,and *** expression levels increased during later meiosis ***,no expression was detected in mature sperm after *** of PGCs with retinoic acid further demonstrated that Piwil1 plays a key role in meiosis during chicken spermatogenesis.