Functional characterization of diacylglycerol kinase gene family in maize(Zea Mays)
作者单位：Heilongjiang Bayi Agricultural University Heilongjiang Remote sensing technology centerHeilongjiang Academy of Agricultural Sciences
会议名称：《2016年全国植物生物学大会》
会议日期：2016年
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 09[农学] 0901[农学-作物学]
关 键 词：maize(zea mays) diacylglycerol kinase bioinformatics analysis abiotic stress
摘 要：Diacylglycerol kinase(DGK) is a kind of phosphokinase involved in the formation of signaling molecule phosphatidic acid(PA) in plants. In this study,seven maize(zea mays) DGK gene family members were identified by bioinformatics analysis,and designated as ZmDGK1-7. The proteins encoded by ZmDGKs had a molecular weight(MWs) between 54.6 and 80.2 k Da,and predicted PI values of 6.08 to 8.74,respectively. Phylogenetic analysis revealed that ZmDGKs fall into three clusters. While all ZmDGKs have a conserved catalytic domain DGKc,only ZmDGK1,ZmDGK4 and ZmDGK5 have the DAG/PE binding domain. Gene expression of ZmDGKs were detected in roots,stem,leaves,and endosperm tissues. ZmDGKs genes were also significantly up-or down-regulated in response to drought,salt and low temperature treatment. We identified maize DGK genes,and conducted bioinformatics and gene expression analysis under several abiotic stresses,which will help us to decipher the functions of maize diacylglycerol kinase family and their roles in maize growth,development and responses to environmental changes.