A conserved Puccinia striiformis effector interacts with wheat NPR1 and reduces induction of pathogenesis-relatedgenes in response to pathogens
作者单位：Department of Plant ScienceUniversity of California State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant ProtectionNorthwest Agriculture and Forestry University Department of Plant PathologyAgriculture University of Hebei Department of Viticulture and EnologyUniversity of CaliforniaDavisCA 95616USA.5.Howard Hughes Medical Institute(HHMI)Chevy Chase
会议名称：《中国植物病理学会2016年学术年会》
会议日期：2016年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Wheat Stripe rust NPR1 Pathogen effector Yeast two-hybrid Bimolecular fluorescence complementation Transgenic barley
摘 要：In Arabidopsis,NPR1 is a key transcriptional co-regulator of systemic acquired *** pathogen challenge,NPR1 translocates from the cytoplasm to the nucleus,where it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related *** a screen of a yeast two-hybrid library from wheat leaves infected with ***,we identified a conserved rust effector that interacts with wheat NPR1 and named it Puccinia NPR1 interactor（PNPi）.PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal *** bimolecular fluorescence complementation assays,we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts.A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and *** barley transgenic lines over expressing PNPi,we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas *** DC3000 *** on these results,we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.