The identification and distribution of gonadotropin releasing hormone in the central nervous system and ovary of each stages in Penaeus monodon
作者单位：South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
会议名称：《2016年中国水产学会学术年会》
会议日期：2016年
关 键 词：GnRH CNS ovarian maturation Penaeus monodon immunocytochemistry qPCR
摘 要：Gonadotropin-releasing hormone（GnRH）plays important roles in *** the present study,we demonstrated the existence of GnRH-like peptides in the central nervous system（CNS）and ovary of each stage in Penaeus monodon using immunocytochemistry and quantitative real-time PCR of c-jun and vitellogenin expression *** immunoreactivity（ir）of GnRH was detected in the of medium-sized neurons located in deutocerebrum,both medium-sized and large-sized neurons in thoracic ganglia and abdominal *** addition,ir-GnRH was observed in the late previtellogenic oocyte and early vitellogenic oocyte.c-jun expression quantity show highest expression in stage Ⅱ,the highest expression of vitellogenin in stage Ⅳ.This finding provides supporting data that ir-GnRH-like peptide may exist in Penaeus monodon,as well as may has a direct and indirect role in promoting ovarian maturation in the early stage.