Isolation and expression analysis of anthocyanin biosynthetic genes in Morus alba L.
作者单位：College of Biotechnology Southwest University
会议名称：《第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会》
会议日期：2016年
学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0905[农学-畜牧学] 09[农学]
摘 要：Anthocyanins from mulberry fruits are used in medicine. However, little anthocyanin can be detected in other tissues and sometimes also mulberry fruits are colorless. The aim of this study was to investigate which gene or genes have the strongest correlation with the anthocyanin biosynthesis. The expression of several anthocyanin synthesis genes were determined in different tissues of two white and two purple fruit cultivars. Genes encoding dihydroflavonol reductase（MaDFR） and anthocyanidin synthase（MaANS） showed a high expression only in fruit tissue of purple-fruit *** the development of mulberry fruits, the anthocyanin content was well correlated with the transcripts abundance of MaDFR, MaANS, and MaCHS（encoding chalcone synthase）. The skin of female mulberry flowers turns red under irradiance because of up-regulated expressions of MaCHS, MaDFR, and MaANS. These three genes may control the anthocyanin biosynthesis in mulberry and up-regulation of them may greatly increase the anthocyanin content. Additional key words: anthocyanidin synthase, chalcone isomerase, chalcone synthase, dihydroflavonol reductase, flavanone-3- hydroxylase, phenylalanine ammonia lyase.